Journal: Journal of Functional Biomaterials

Article Title: Corncob Cellulose Scaffolds: A New Sustainable Temporary Implant for Cartilage Replacement

doi: 10.3390/jfb13020063

Figure Lengend Snippet: Cytotoxicity assay with L929 mouse fibroblasts through ( a ) indirect contact (MTT protocol) ( n = 5) and ( b ) direct contact (20× amplification) of negative and positive controls and the scaffolds produced with PCL and: 1% wood cellulose (WC_1%); 2% wood cellulose (WC_2%); 1% corncob cellulose (CC_1%); 2% corncob cellulose (CC_2%). Scale bar: 100 μm. p < 0.001 (***).

Article Snippet: The biocompatibility of the manufactured scaffolds was assessed using L929 mouse fibroblasts (ATCC number CCL-1) and following the ISO 10993-5 and ISO 10993-12 guidelines [ ].

Techniques: Cytotoxicity Assay, Amplification, Produced

Journal: Molecules

Article Title: The Antioxidant, Cytotoxic and Antimicrobial Potential of Phenolic Acids-Enriched Extract of Elicited Hairy Roots of Salvia bulleyana

doi: 10.3390/molecules27030992

Figure Lengend Snippet: Cytotoxic effect of S. bulleyana extract towards the LoVo, AGS, HeLa and L929 cell lines. The cell viability was calculated for four experiments, including three replicates for each compound. Complete RMPI-1640 medium (cRPMI) was used as a positive control of cell viability (100% viable cells) and 0.03% H 2 O 2 as a negative control of cell viability (5% for LoVo, 2.2% for AGS, 3.2% for HeLa, 3.3% for L929). *—determination of statistical significance for untreated cells vs. cells treated with tested plant extract for the same cell line according to the Kruskal–Wallis test ( p < 0.05).

Article Snippet: In vitro cytotoxicity testing was performed using human HeLa (CCL-2, American Type Culture Collection (ATCC), Rockville, MD, USA) cervix adenocarcinoma epithelial cells, human AGS (CRL-1739, ATCC, Rockville, MD, USA) gastric adenocarcinoma epithelial cells, human colon epithelial LoVo (CCL-229™, ATCC, Rockville, MD, USA) and normal mouse fibroblasts L929 (LGC Standards, Middlesex, UK).

Techniques: Positive Control, Negative Control

Journal: Molecules

Article Title: Effect of Currently Available Nanoparticle Synthesis Routes on Their Biocompatibility with Fibroblast Cell Lines

doi: 10.3390/molecules27206972

Figure Lengend Snippet: Mouse fibroblast’s cell morphology exposed to control group prepared by water on first day, 15th day, 31st day, 41st day and 51st day, showing normally large, elongated flat cells with cytoplasm ( a , e , i , m , q ). Mouse fibroblast’s cell morphology exposed to experimental group of titanium nanoparticles prepared by Bacillus Subtilus on first day, 15th day, 31st day, 41st day and 51st day showing normally large, elongated flat cells with cytoplasm ( b , f , j , n , r ). Mouse fibroblast’s cell morphology exposed to experimental group of titanium nanoparticles prepared by Cassia fistula on first day, 15th day, 31st day, 41st day and 51st day, showing initiation of pore formation ( c ), increased pore formation ( g ), increased pore formation and mild degradation ( k ), increased pore formation and mild degradation ( o ) and loss of normal spindle shape ( s ). Mouse fibroblast’s cell morphology exposed to experimental group of titanium nanoparticles prepared by hydrothermal heating on the first day, 15th day, 31st day, 41st day and 51st day, showing slight degradation ( d ), increased pore formation and degradation ( h ), greater disruption ( l ), complete loss of cell symmetry ( p ) and entire loss of normal size, shape and symmetry of cell ( t ).

Article Snippet: Mouse fibroblast cells (L929 (ATCC HTB-85, Manassas, VA, USA)) were grown in 25 cm 2 vented cell culture flasks in a humified incubator at 37 °C with 5% CO 2 .

Techniques: Control, Disruption